学习及工作经历

研究领域及兴趣

化学与生物传感；病原体检测新方法开发及应用；功能核酸生物传感器

主持课题：

中国博士后科学基金，面上项目（一等），2022M710152，基于G-四链体和CRISPR/Cas13aRNA高灵敏检测新方法研究，2023.01-2024.12，12 万元，主持；

代表性论文：

（1）Tao Li^#, Dongjuan Chen^#,Xiaoling He, Zheyu Li, Runchen Li, Bingxin Zheng, Rui Hu, Jiang Zhu, Ying Li^*, Yunhuang Yang^*. Leveraging Cas13a’s trans-cleavage on RNA G-quadruplexes for amplification-free RNA detection, Chemical Communications, 2024, 60 10.1039/d3cc06238d. (IF=4.9, 中科院2区)

（2）Tao Li^#, Rui Hu^#, Jianbo Xia^#, Zhichen Xu, Dongjuan Chen, Jinou Xi, Bi-Feng Liu, Jiang Zhu, Ying Li^*, Yunhuang Yang, Maili Liu^*. G-triplex: A new type of CRISPR-Cas12a reporter enabling highly sensitive nucleic acid detection, Biosensors and Bioelectronics, 2021, 187, 113292. (IF=12.6, 中科院1区)

（3）Tao Li^#, Zhaowei Zhang^#, Yu Wang, Ying Li, Jiang Zhu, Rui Hu*, Yunhuang Yang*, Maili Liu. Quantitative proteomic analysis for high- and low-aflatoxin-yield Aspergillus flavus strains isolated from natural environments, Frontiers in Microbiology, 2021, 12, 741875. (IF=5.2, 中科院2区)

（4）Tao Li^#, Gaozhi Ou^#, Xuliang Chen, Zheyu Li, Rui Hu, Ying Li^*, Yunhuang Yang^*, Maili Liu. Naked-eye based point-of-care detection of E. coli O157: H7 by a signal amplified microfluidic aptasensor, Analytica Chimica Acta, 2020, 1130, 20e28. (IF=6.2, 中科院2区)

（5）Ying Li^{*, #}, Tao Li^#, Bi-Feng Liu, Rui Hu, Jiang Zhu, Ting He, Xin Zhou, Conggang Li, Yunhuang Yang, Maili Liu*. CRISPR-Cas12a trans-cleaves DNA G-quadruplexes, Chemical Communications, 2020, 56, 12526-12529. (IF=4.9, 中科院2区)

（6）Zhichen Xu^#, Dongjuan Chen^#, Tao Li, Jiayu Yan, Jiang Zhu, Ting He, Rui Hu, Ying Li^*, Yunhuang Yang, Maili Liu. Microfluidic space coding for multiplexed nucleic acid detection via CRISPR-Cas12a and recombinase polymerase amplification, Nature Communications, 2022, 13(1), 6480. (IF=16.6, 中科院1区)

（7）Zheyu Li^#, Rui Hu^#, Tao Li, Jiang Zhu, Huijuan You, Yiwei Li, Bi-Feng Liu, Conggang Li, Ying Li^*, Yunhuang Yang^*. A TeZla micromixer for interrogating the early and broad folding landscape of G-quadruplex via multistage velocity descending, PNAS, 2024, 121(4): e2315401121. (IF=11.1, 中科院1区)

（8）Yin Zhao, Dongjuan Chen, Zhichen Xu, Tao Li, Jiang Zhu, Rui Hu, Guoyong Xu*, Ying Li*, Yunhuang Yang*, Maili Liu. Integrating CRISPR-Cas12a into a Microfluidic Dual-Droplet Device Enables Simultaneous Detection of HPV16 and HPV18, Analytical Chemistry, 2023, 95(6): 3476-3485. (IF=7.4, 中科院1区)

（9）Jiayu Yan, Zhichen Xu, Hu Zhou, Tao Li, Xincheng Du, Rui Hu, Jiang Zhu, Gaozhi Ou*, Ying Li, Yunhuang Yang. Integration of CRISPR/Cas12a and Multiplexed RPA for Fast Detection of Gene Doping, Analytical Chemistry, 2022, 94(47): 16481-16490. (IF=7.4, 中科院1区)

（10）Ying Li^*, Pengchao Zhang, Tao Li, Rui Hu, Jiang Zhu, Ting He, Yunhuang Yang, Maili Liu. A virtual-droplet system for sensing MMP9 activity of single suspended and adhered cancer cells, Sensors and Actuators B: Chemical, 2020, 308, 12449. (IF=8.4, 中科院1区)

发明专利：

（1）李颖，李涛，胡锐，杨运煌，周欣，刘买利，一种快速可视化的溶血性大肠杆菌O157：H7检测方法，中国专利 2019，专利号：ZL201910416351.3;

（2）李颖，李涛，杨运煌，胡锐，刘买利，一种新型的核酸检测方法，中国专利 2020，专利号：ZL202010983248.X。

（3）李颖，徐志辰，杨运煌，李涛，用于HPV分型的多靶标核酸检测试剂盒和检测方法，中国专利 2021，专利号：ZL202111472349.1;

（4）李颖，徐志辰，杨运煌，李涛，Nucleic acid analysis kit and method for multiplexed HPV subtyping，2022，PCT已获批: PCT/CN2022/114122。